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)Matsuda, Asahi*; Takahashi, Satoru*; Kawabata, Kuniaki; Oda, Shoji*; Kaneko, Shunichi*
Denki Gakkai Rombunshi, D, 139(4), p.424 - 432, 2019/04
Yasuda, Takako*; Oda, Shoji*; Asaka, Tomomi*; Funayama, Tomoo; Yokota, Yuichiro; Muto, Yasuko*; Ikeda, Hiroko; Kobayashi, Yasuhiko; Mitani, Hiroshi*
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 85, 2015/03
In this present study, we examined the effects of heavy carbon-ions on development in pre-implantation period utilizing medaka blastula stage embryos (st. 11: blastderm diameter is about 500 
m). We performed targeted irradiation by carbon-ion micro-beam (diameters of 120, 180 m) to a central parts of blastoderm and observed the abnormalities during development compared with whole-body irradiated embryos. As a results, retardation and characteristic malformed eyes were observed during development when blastoderm cells were partially irradiated, However, more than half of 50 Gy-irradiated embryos (area size=120 m diameter) could hatch normally in contrast to all embryos with 2 Gy of whole-body irradiation being lethal before hutching.
Matsuda, Asahi*; Takahashi, Satoru*; Kawabata, Kuniaki; Oda, Shoji*; Kaneko, Shunichi*
no journal, ,
Medaka (Oryzias latipes) has genetic diversity, and analyzing its behavior in a group is very important for elucidation of diversity of organisms. However, conventional method of visual measurement has some problems. For example, the time cost and human error. In this paper, we proposed a method of behavior measurement of medaka in a group based on an image instrumentation. Our proposed method consists of four processes: pre-processing, particle filter, orientation code matching using by central differences with multi-templates, and detection of target position. Finally, we examined the effectiveness of our proposed method through an experimentation.
